FARIA ZAINAB2025-04-302025-04-302023-06-15https://repository.kinnaird.edu.pk/handle/123456789/199Peganum Harmala has been used in traditional medicine as a disinfectant and pain reliever for a long time. It is extensively dispersed and utilized as a medicinal plant and also has antibacterial, antifungal, antiviral, antioxidant, anti-diabetic, anti cancer, and anti-leishmanial effects. The aim of this study is to confirm the cytotoxicity and wound healing potential of the aqueous extract of Peganum harmala. The wound healing capacity of Peganum harmala extracts at different doses (50 and 100 mg/kg body weight) was assessed using the wound index, sandwich enzyme-linked immunosorbent assay (ELISA), antioxidant estimation, and histological examination. The maximum potential for wound healing was suggested at extract doses of 50 mg per kg by raising levels of vascular endothelial growth factor (VEGF). Additionally, a dose of 100 mg of Peganum harmala plant extract per kilogram demonstrated remarkable wound healing capacity with elevated VEGF levels. The cytotoxic potential of Peganum harmala extracts on HEK cell line at 570 nm was assessed using the MTT test. The Peganum harmala plant extract was shown to have decreased cytotoxicity or increased cytoprotectivity in the aqueous extracts, indicating that it is safe to use with an IC50 against the HEK cell line. Based on the aforementioned findings, it is strongly advised as a possible continuation of the current effort to extract, identify, and purify phytochemicals from Peganum harmala extractsen-USThesis