MPhil Students

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Browsing MPhil Students by Author "MAHNOOR SAFDAR"

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    UTILIZATION OF FOOD WASTE TO PRODUCE α AMYLASE AND SYNTHESIS OF α AMYLASE- NANOPARTICLES COMPOSITE FOR THE DEGRADATION OF TEXTILE DYE
    (0024-01-18) MAHNOOR SAFDAR
    Green synthesis of nanoparticles has bought the trend of using biomaterials to synthesize a variety of metallic nanoparticles. The current study reports the use of α amylase crude extract obtained by Aspergillus niger to synthesize copper oxide nanoparticles. Characterization of CuO NPs was carried out using UV-Vis spectroscopy, scanning electron microscopy (SEM), Energy dispersive x-ray (EDX) spectroscopy and Fourier transform infrared spectroscopy, while the effectiveness of CuO NPs as catalytic agents for degradation of textile dye was evaluated. FTIR analysis shows protein molecules were responsible for capping and stabilization of nanoparticles while EDX analysis showed the presence of oxygen with copper. TEM analysis showed that particles size were less than 20nm. 100 µg/ml and 150 µg/ml doses of CuO NPs were found effective to degrade the red dye of different concentrations (1ppm, 2ppm, 5ppm, 10ppm, 15ppm, 20ppm) at different time intervals. 100% dye degradation was achieved at dose of 100 µg/ml dose at 1ppm of dye concentration. This study has demonstrated utilization of food waste into production of a significant and cost-effective product i.e. α amylase CuO NP composit