Browsing by Author "AYESHA SHAHZAD"

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    TOXICITY PROFILING OF CARICA PAPAYA LEAVES
    (DEPARTMENT OF ZOOLOGY KINNAIRD COLLEGE FOR WOMEN UNIVERSITY LAHORE,, 2023-06-13) AYESHA SHAHZAD
    The common name of papaya plant is papita. The scientific name is Carica. papaya. It is soft stem long plant. The peel, pulp and seed of the papaya contains phytochemical and the carotenoids. The plant extract was done by drying the leaves of papaya plant in shade and stock solution was prepared (1mg/ml, 2mg/ml, 10mg/ml, 50mg/ml, 100mg/ml). three bacterial strains were used (Streptococcus pneumonia, Staphylococcus aureus, Pseudomonas aeruginosa) for the determination of antibacterial activity, and for the determination of antifungal activity aspergillus niger was used. For the determination of antioxidants DPPH and catalase assay was done. For cytotoxicity nalysis MTT assay was performed. The DPPH assay shows the dose P10 is show significant results, P50 and P100 show highly significant result. The catalase assay shows the dose P10(10mg/ml,5.5±0.19), P50(50mg/ml, 7.2±0.055) and P100 (100mg/ml, 11±0.14) show significant result as compared to control. The antibacterial activity of papaya leave extract against the S.pneumonia, the results shown in dilution 20 µl of dose P10 (10 mg /ml, 1.5333±0.120). and for the P. aeruginosa, the best results were shown in dilution 40 µl of dose P50 (50 mg/ml, 2.433±0.067). for the S.aereus, the best results were shown in 40 µl of dose P100(100mg/ml, 2.666±0.033). for the determination of antifungal activity The antifungal properties of papaya leave extract against the A.niger. The best result 40 µl show abundant zone of inhibition of dose P100(100mg/ml, 3.033±0.033). To check the toxicity profiling by MTT assay of the C. papaya leaves, shows no toxcicity. Our research concludes that the ethanolic papaya leave extract have ability to inhibit the growth of bacteria and fungus, and have antioxidant capacity.
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    TOXICITY PROFILING OF CARICA PAPAYA LEAVES
    (DEPARTMENT OF ZOOLOGY, 2023-06-15) AYESHA SHAHZAD
    The common name of papaya plant is papita. The scientific name is Carica. papaya. It is soft stem long plant. The peel, pulp and seed of the papaya contains phytochemical and the carotenoids. The plant extract was done by drying the leaves of papaya plant in shade and stock solution was prepared (1mg/ml, 2mg/ml, 10mg/ml, 50mg/ml, 100mg/ml). three bacterial strains were used (Streptococcus pneumonia, Staphylococcus aureus, Pseudomonas aeruginosa) for the determination of antibacterial activity, and for the determination of antifungal activity aspergillus niger was used. For the determination of antioxidants DPPH and catalase assay was done. For cytotoxicity nalysis MTT assay was performed. The DPPH assay shows the dose P10 is show significant results, P50 and P100 show highly significant result. The catalase assay shows the dose P10(10mg/ml,5.5±0.19), P50(50mg/ml, 7.2±0.055) and P100 (100mg/ml, 11±0.14) show significant result as compared to control. The antibacterial activity of papaya leave extract against the S.pneumonia, the results shown in dilution 20 µl of dose P10 (10 mg /ml, 1.5333±0.120). and for the P. aeruginosa, the best results were shown in dilution 40 µl of dose P50 (50 mg/ml, 2.433±0.067). for the S.aereus, the best results were shown in 40 µl of dose P100(100mg/ml, 2.666±0.033). for the determination of antifungal activity The antifungal properties of papaya leave extract against the A.niger. The best result 40 µl show abundant zone of inhibition of dose P100(100mg/ml, 3.033±0.033). To check the toxicity profiling by MTT assay of the C. papaya leaves, shows no toxcicity. Our research concludes that the ethanolic papaya leave extract have ability to inhibit the growth of bacteria and fungus, and have antioxidant capacity.