TOXICITY PROFILING OF CARICA PAPAYA LEAVES
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Date
2023-06-15
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DEPARTMENT OF ZOOLOGY
Abstract
The common name of papaya plant is papita. The scientific name is Carica. papaya.
It is soft stem long plant. The peel, pulp and seed of the papaya contains
phytochemical and the carotenoids. The plant extract was done by drying the leaves of
papaya plant in shade and stock solution was prepared (1mg/ml, 2mg/ml, 10mg/ml,
50mg/ml, 100mg/ml). three bacterial strains were used (Streptococcus pneumonia,
Staphylococcus aureus, Pseudomonas aeruginosa) for the determination of
antibacterial activity, and for the determination of antifungal activity aspergillus niger
was used. For the determination of antioxidants DPPH and catalase assay was done.
For cytotoxicity nalysis MTT assay was performed. The DPPH assay shows the dose
P10 is show significant results, P50 and P100 show highly significant result. The
catalase assay shows the dose P10(10mg/ml,5.5±0.19), P50(50mg/ml, 7.2±0.055) and
P100 (100mg/ml, 11±0.14) show significant result as compared to control. The
antibacterial activity of papaya leave extract against the S.pneumonia, the results
shown in dilution 20 µl of dose P10 (10 mg /ml, 1.5333±0.120). and for the P.
aeruginosa, the best results were shown in dilution 40 µl of dose P50 (50 mg/ml,
2.433±0.067). for the S.aereus, the best results were shown in 40 µl of dose
P100(100mg/ml, 2.666±0.033). for the determination of antifungal activity The
antifungal properties of papaya leave extract against the A.niger. The best result 40 µl
show abundant zone of inhibition of dose P100(100mg/ml, 3.033±0.033). To check
the toxicity profiling by MTT assay of the C. papaya leaves, shows no toxcicity. Our
research concludes that the ethanolic papaya leave extract have ability to inhibit the
growth of bacteria and fungus, and have antioxidant capacity.