EFFECT OF HERMAL PLANT ON CELLULAR VIABILTY AND SKIN REGENERATION ABILITY OF ACID BURN RATS
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Date
2023-06-15
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DEPARTMENT OF ZOOLOGY KINNAIRD COLLEGE FOR WOMEN UNIVERSITY LAHORE,
Abstract
Peganum Harmala has been used in traditional medicine as a disinfectant and pain
reliever for a long time. It is extensively dispersed and utilized as a medicinal plant
and also has antibacterial, antifungal, antiviral, antioxidant, anti-diabetic, anti cancer, and anti-leishmanial effects. The aim of this study is to confirm the
cytotoxicity and wound healing potential of the aqueous extract of Peganum
harmala. The wound healing capacity of Peganum harmala extracts at different
doses (50 and 100 mg/kg body weight) was assessed using the wound index,
sandwich enzyme-linked immunosorbent assay (ELISA), antioxidant estimation,
and histological examination. The maximum potential for wound healing was
suggested at extract doses of 50 mg per kg by raising levels of vascular endothelial
growth factor (VEGF). Additionally, a dose of 100 mg of Peganum harmala plant
extract per kilogram demonstrated remarkable wound healing capacity with
elevated VEGF levels. The cytotoxic potential of Peganum harmala extracts on
HEK cell line at 570 nm was assessed using the MTT test. The Peganum harmala
plant extract was shown to have decreased cytotoxicity or increased
cytoprotectivity in the aqueous extracts, indicating that it is safe to use with an IC50
against the HEK cell line. Based on the aforementioned findings, it is strongly
advised as a possible continuation of the current effort to extract, identify, and
purify phytochemicals from Peganum harmala extracts