EFFICACY OF PROBIOTICS SUPPLEMENTATION ON GROWTH PERFORMANCE, IMMUNOLOGICAL AND HAEMATOLOGICAL PARAMETERS OF LABEO ROHITA
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Date
2023-06-15
Journal Title
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DEPARTMENT OF ZOOLOGY KINNAIRD COLLEGE FOR WOMEN UNIVERSITY LAHORE,
Abstract
To access water soluble probiotic supplementation mixture of Saccharomyces cerevisiae, Bacillus
subtilis, Bacillus licheniformis, Trichoderma long brachium on growth, hematological and
immunological parameters of Labeo rohita. Fish is divided into two groups, control and
experimental. Control group fed only with feed. Experimental group fed with diet + water soluble
probiotics. After 60 days there was significant increase in growth of fishes, protein efficiency
ratio (PER) and feed conversion ratio (FCR).
In evaluating hematological parameters includes hemoglobin (Hb), hematocrit (Ht) Red bloodcell
count (RBC), Mean Corpuscular Volume (MCV), Mean Corpuscular hemoglobin concentration
(MCHC) and Mean Corpuscular hemoglobin (MCH),. As compared to control (2.81 ± 0.03) the
red blood cell count (RBC) was significantly higher at treatment group (3.67 ± 0.22) for 60 days
and (P <0.05) which were highly significant. The highest hemoglobin percent was recorded at
experimental group for 60 days (5.88 ± 0.05) and (P <0.05), whereas the lowest was in the
control group (5.45±0.04).The experimental group had the highest Mean Corpuscular
hemoglobin concentration values (22.87 ± 0.06) and (P <0.05) which was highly significant than
the control group which have the lowest value (19.76 ± 0.04) for 60 days.
The immunological parameters include leucocytes count and serum lysozyme activity. The total
amount of leucocytes in the fish diet with experimental group was substantially higher (P <0.05)
than the fish in the control group. Similarly, the experimental group (7.89 ± 0.05 U ml 1
) had
considerably higher serum lysozyme activity than the control group (4.90 ±0.05 U ml 1
). The
results showed that water soluble probiotics exert a significant influence on survival, growth,
hematological, and immunological parameters of Labeo rohita.